Rabbit anti-ATM Recombinant Monoclonal Antibody [BLR359N]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 2550 and 2600 of human Serine-protein kinase ATM using the numbering given in entry NP_000042.3 (Gene ID 472).
Additional Product Information
Ataxia telangiectasia, mutated (ATM) is the gene responsible for the neurodegenerative disease ataxia telangiectasia (AT). AT is characterized by neurodegeneration, immune dysfunction, sensitivity to DNA damage, and cancer predisposition. ATM is a protein kinase central to the DNA damage response. In response to DNA double-strand breaks (DSBs), ATM initiates a signaling cascade that involves the phosphorylation of a multitude of substrates which include p53, BRCA1, p53 binding protein, CHK2, RAD9, RAD17, and MDM4.
Alternate Names
AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated; ATC; ATD; ATDC; ATE; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.