Rabbit anti-MCU Recombinant Monoclonal Antibody [BLR355N]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 300 and 350 of human Mitochondrial calcium uniporter MCU using the numbering given in entry NP_612366.1 (Gene ID 90550).
Additional Product Information
MCU is a channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix. MCU is involved in several processes, including cellular response to calcium ion starvation, positive regulation of mitochondrial calcium ion concentration, and positive regulation of mitochondrial fission. [taken from NCBI Entrez Gene (Gene ID: 90550)].
Alternate Names
C10orf42;calcium uniporter protein, mitochondrial;CCDC109A;coiled-coil domain-containing protein 109A;HsMCU
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.