Rabbit anti-NP95/UHRF1 Recombinant Monoclonal Antibody [BLR377N]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 1 and 50 of human E3 ubiquitin-protein ligase UHRF1 using the numbering given in entry NP_001041666.1 (Gene ID 29128).
Additional Product Information
NP95/UHRF1 was originally identified as ICBP90 (inverted CCAAT box-binding protein of 90 kDa) in a one-hybrid screen for proteins that bind the topoisomerase IIalpha gene promoter. NP95/UHRF1 is a heterochromatin binding protein that directly interacts with histones and has proposed roles in the DNA damage response, heterochromatin formation, and cell replication. Structurally, NP95/UHRF1 contains an ubiquitin-like domain (NIRF_N), a plant homeodomain (PHD), a SRA domain, and a RING domain. The RING domain imparts ubiquitin E3 ligase activity that is histone-specific. This E3 ligase activity is proposed to be important to tumor growth. NP95/UHRF1 expression begins in the G1/S phase and continues through mitosis. It has been shown to be required for entry from G1/G0 as well as the G1/S transition in some cell types. NP95/UHRF1 has been found to be part of the pericentric heterochromatin duplication body (pHDB) and involved in the replication and formation of heterochromatin.
Alternate Names
E3 ubiquitin-protein ligase UHRF1;hNP95;hUHRF1;huNp95;ICBP90;inverted CCAAT box-binding protein of 90 kDa;Np95;nuclear phosphoprotein 95;nuclear protein 95;nuclear zinc finger protein Np95;RING finger protein 106;RING-type E3 ubiquitin transferase UHRF1;RNF106;TDRD22;transcription factor ICBP90;ubiquitin-like PHD and RING finger domain-containing protein 1;Ubiquitin-like-containing PHD and RING finger domains protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.