Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5] - BSA-Free
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
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Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155). Recombinant antibody was purified from cell culture supernatant.
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
Additional Product Information
KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.
Alternate Names
E3 SUMO-protein ligase TRIM28;KAP1;KAP-1;KRAB [Kruppel-associated box domain]-associated protein 1;KRAB-associated protein 1;KRAB-interacting protein 1;KRIP-1;nuclear corepressor KAP-1;PPP1R157;protein phosphatase 1, regulatory subunit 157;RING finger protein 96;RING-type E3 ubiquitin transferase TIF1-beta;RNF96;TF1B;TIF1B;TIF1-beta;transcription intermediary factor 1-beta;transcriptional intermediary factor 1-beta;Tripartite motif-containing protein 28
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.