Rabbit anti-SOCS3 Recombinant Monoclonal Antibody [BLR382N] - BSA-Free
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
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Immunogen was a peptide representing a region between residue 207 and 220 of human Suppressor of cytokine signaling 3 (aka SOCS3) using the numbering given in entry NP_003946.3 (Gene ID 9021).
Additional Product Information
SOCS3 is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of SOCS3 is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. SOCS3 can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of SOCS3 suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [taken from NCBI Entrez Gene (Gene ID: 9021)]
Alternate Names
ATOD4;CIS3;CIS-3;Cish3;cytokine-inducible SH2 protein 3;SOCS-3;SSI3;SSI-3;STAT-induced STAT inhibitor 3;suppressor of cytokine signaling 3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.